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ผลงานตีพิมพ์ในวารสารวิชาการInsecticidal and growth inhibitory effects of some thymol derivatives on the beet armyworm, Spodoptera exigua (Lepidoptera:Noctuidae) and their impact on detoxification enzymesผู้แต่ง: Anchulee Pengsook, Sorachat Tharamak, Kanta Keosaeng, Opender Koul,  Dr.Vasakorn Bullangpoti, Professor , Nutchaya Kumrungsee, Dr.Wanchai Pluempanupat, Associate Professor , วารสาร:    
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ผลงานตีพิมพ์ในวารสารวิชาการBioefficacy of piper ribesioides (Piperaceae: Piperales) extracts and isolated piperine against Aedes aegypti (Diptera:Culicidae) larvae and their impact on detoxification enzymesผู้แต่ง:Kumrungsee, N., Wongsa, N., Ruttanakum, D., Ratwatthananon, A., Pengsook, A., Manaprasertasak, A., Dr.Wanchai Pluempanupat, Associate Professor, Dr.Vasakorn Bullangpoti, Professor, Koul, O., วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการEffects of the botanical insecticide thymol on biology of a braconid, Cotesia plutellae(Kurdjumov), parasitizing the diamondback moth, Plutella xylostella L.ผู้แต่ง:Paraporn Yotavong, Dr.Boonsatien Boonsoong, Associate Professor , Dr.Wanchai Pluempanupat, Associate Professor, Opender Koul, Dr.Vasakorn Bullangpoti, Professor, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการChrysoeriol isolated from Melientha suavis Pierre with activity against the agricultural pest Spodoptera lituraผู้แต่ง:Ruttanaphan, T., Thitathan, W., Dr.Narisara Piyasaengthong, Assistant Professor , Nobsathian, S., Dr.Vasakorn Bullangpoti, Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการA plant-based extract mixture for controlling Spodoptera litura (Lepidoptera: Noctuidae)ผู้แต่ง:Yooboon, T, Pengsook, A, Ratwatthananon, A, Dr.Wanchai Pluempanupat, Associate Professor, Dr.Vasakorn Bullangpoti, Professor, วารสาร: |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Toxicity of 4,11-Selinnadien-3-one from Nutsedge (Cyperus rotundus L.) Tuber Extracts to Diamondback Moth Larvae (Plutella xylostella L.), Detoxification Mechanisms and Toxi ผู้เขียน:ดร.สุรพล วิเศษสรรค์, รองศาสตราจารย์, Mantana Milne, John Milne สื่อสิ่งพิมพ์:pdf AbstractTubers of nutgrass (Cyperus rotundus L.) were collected from various locations of Thailand during the summer and rainy seasons. Toxicity against the diamondback moth (Plutella xylostella L.) was observed using different concentrations of the active compound, 4,11-selinnadien-3-one. The toxic effects were also determined on mice (Mus musculus), fish (Poecillia reticulata) and bee larvae (Apis florea). It was found that the active principle of nut grass was higher in summer than that in rainy season by ca. 2 folds. This active principle varied according to geographical vareas with Chanthaburi and Chaing Mai producing the highest amounts of 4,11-selinnadien-3-one (0.13-0.16% ai. yield) compared with the other. The LC50 against 2nd–3rd instar larvae of diamondback moth were 7-12 ppm. Detoxification enzyme activities as well as synergistic effects revealed that monooxygenase, esterases and some degrees of glutathione-S-transferase played a role in detoxification. Furthermore, synergists, PB and TPP, could raise the effectiveness of the active principle up to ca. 2-6 fold. At 2,000 ppm of 4,11-selinnadien-3-one, exposed mice showed no sign of acute dermal, acute oral or eye irritation effects. However, the active principle was toxic to other non target organisms with LC50 of 28.01 ppm and 10.8 ppm to 1-month old guppies and bee larvae, respectively. |
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ที่มา:กองทุนสนับสนุนการวิจัยและศูนย์พันธุวิศวกรรมและเทคโนโลยีชีวภาพแห่งชาติหัวเรื่อง:ผลของสารสกัดจากตะไคร้หอมและสารสกัดจากสะเดาต่อเอนไซม์ทำลายพิษในเห็บสุนัข หัวหน้าโครงการ:ดร.สุรพล วิเศษสรรค์, รองศาสตราจารย์ |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Root Extract from Derris (Derris elliptica Benth) on Mortality and Detoxification Enzyme Levels in the Diamondback Moth Larvae (Plutella xylostella Linn.)) ผู้เขียน:ดร.สุรพล วิเศษสรรค์, รองศาสตราจารย์, มัณฑนา มิลน์ สื่อสิ่งพิมพ์:pdf AbstractTwo types of ethanol extraction methods, Soxhlet and stirring soaking, were carried out. The rotenone content determined by the HPLC was 8.6 % w/w for the former method compared to 5.2% w/w for the latter one. Third instar larvae of the diamond back moth gave LD50 of 24.25 PPM and 89.07 PPM for the Soxhlet and stirring methods, respectively. Triphenyl phosphate (TPP) and Piperonylebutoxide (PB) reduced the LD50 upto ca. 4 folds and also significantly lowered the number of larvae in the field. The optimal medium for the detoxification enzymes, esterase, GSH-S-transferses and monooxygenases, was found to contain 0.1 M phosphate buffer pH 7.5 with 10 mM glutathione (reduced forms), 1 mM EDTA and 50% w/w PVPP. Protein concentrations (BSA as a standard protein) between 50-100 mg protein/g larvae/ml extracted from 2 – 4 instar larvae were used for all enzyme assays. Derris extracts induced ca. 10-20% of all enzyme activities. By adding TPP to the extracts, esterase activity was reduced by 20%. The coefficient of correlation, r2 (mortality against esterase activity), was ca. 0.9. The addition of DEM showed a lower in r2 value (0.62 – 0.77) (mortality against GSH-S-transferases activity). The highest fluctuation of r2 (0.48 – 0.97) (mortality against monooxygenases activity) was observed by the addition of PB to both extracts. |